DOI: 10.14704/nq.2018.16.6.1627

Effect of Sanpian Decoction on CGRP, 5-HT and IL-1β in Peripheral Blood of Migraine Model Rats

Yan Liu, Yonglie Zhao, Jinmin Liu


This study investigates the effect of Sanpian Decoction on the calcitonin gene related peptide (CGRP), 5-hydroxytryptamine (5-HT) and interleukin-1β (IL-1β) in peripheral blood of migraine model rats. Methods: A total of 24 healthy SD rats of SPF grade are randomly divided into 4 groups: saline control group, migraine model group and sumatriptan succinate group, Sanpian Decoction group. The experimental migraine animal model is replicated by subcutaneous injection of nitroglycerin on the back of the rats’ neck for 3 weeks. After the second injection of nitroglycerin, drug intervention is given. One week later, the materials are drawn 2 hours after the third injection of nitroglycerin, and the contents of CGRP, 5-HT and IL-1β in peripheral blood of rats in each group are measured by Elisa method. Results: The content of serum CGRP in migraine model group increases, which is different from that in saline control group, the difference is statistically significant (P<0.05); The serum CGRP of migraine model group is compared with that of sumatriptan succinate group and Sanpian Decoction group (P < 0.05); The serum CGRP of sumatriptan succinate group is compared to that of Sanpian Decoction group (P > 0.05); The content of 5-HT in the serum of migraine model group decreases, but there is no statistically significant difference between the groups (P > 0.05); The content of IL-1β in the serum of migraine model group increases, but there is no statistically significant difference between the groups (P > 0.05). Conclusions: Sanpian Decoction can reduce the expression of CGRP in peripheral blood of migraine rats induced by nitroglycerin, increase the content of 5-HT in its peripheral blood, and reduce the content of IL-1β in its peripheral blood, which may also inhibit the transmission of pain information by improving the inflammatory response induced by CGRP.


Migraine, Animal Model, Sanpian Decoction, Peripheral Blood, CGRP, 5-HT, IL-1β

Full Text:



Bolay H, Reuter U, Dunn A, Huang Z, Boas D, Moskowitz MA. Intrinsic brain activity triggers trigeminal meningeal afferents in a migraine mode. Nature Medicine 2002; 8(2): 136-42.

Gao X, Lu X. Research progress in the treatment of migraine headache in the past ten years. Clinical Journal of Traditional Chinese Medicine 2015; 10(27): 1480-82.

Guo HL Wang JC, Hu LJ, Zhao XJ, Gao WJ, Jiang L. the comparison of the anti-inflammatory and analgesic effects of different processed products of Cyperus. Journal of Jiangxi University of Traditional Chinese Medicine 2017; 29(1): 74-83.

Lei HE, Yong-rui BAO, Xian-sheng MENG, Shuai WANG, Tian-jiao LI, Li FU. Potential mechanism of Chuanxiong phenolic acid components for rat migraine by promoting blood circulation and removing blood stasis, Central South Pharmacy 2018; 16(1): 45-49.

Messlinger K. Migraine: where and how does the pain originate. Experimental Brain Research 2009; 196(1): 179-93.

Pietrobon D, Striessnig J. Neurobiology of migraine. Nature Reviews Neuroscience 2003; 4(5): 386-98.

Raddant AC, Russo AF. Calcitonin gene-related peptide in migraine: Intersection of peripheral inflammation and central modulation. Expert Reviews in Molecular Medicine 2011; 13: e36.

Russo AF. Calcitonin Gene-Related Peptide (CGRP): a new target for migraine. Annual Review of Pharmacology and Toxicology 2015; 55: 533-52.

Wan J, Huang H, Polytechnic W. Anti-inflammatory and analgesic effects of different extract fractions from brassica alba. Asia-Pacific Traditional Medicine 2014, 10(5): 39-40.

Wang D, Yu SY. The study of the effect of estrogen on migraine. Chinese Journal of Modern Drug Application 2015; 9(5): 251-53.

Zhang W. Clinical Application and Pharmacological Actions of Ligustrazine Chuanxiong. China Health Standard Management 2015; 6(26): 117-18.

Zhao Y, Wang Y, Yao Z, Yue G. Effect of Xiongzhi Dilong Decoction on NO, NOS and cGMP in Migraine Model with Wind-heat Syndrome. Journal of Integrative Medicine on Cardio-/Cerebrovascular Disease 2015; 10(8): 1165-68.

Zhou ZL, Liu YH. Study on antidepressant effect and mechanism by Cyperus rotundus extracts. Chinese Journal of Experimental Traditional Medical Formulae 2012; 18(7): 191-93.

Zhu Y, Baoli LI, Hongsheng MA, Liu Y, University Y. Research progress of Angelica dahurica for extracting effective components, pharmacological action and clinical application. China Medical Herald 2014; 11(31): 159-66.

Supporting Agencies

| NeuroScience + QuantumPhysics> NeuroQuantology :: Copyright 2001-2019