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Home > Archives > Volume 20, No 5 (2022) > Article

DOI: 10.14704/nq.2022.20.5.NQ22146

First Molecular Study and Sequencing of Cryptosporidium spp. Isolated from Human, Cattle and Sheep in Wasit Province, Iraq

Noor J. Hussein and May N. Khadim

Abstract

The current study aimed to investigate Cryptosporidium spp. that infect humans and some animal species as well as to compare among various isolates obtained. A total of 96 human stool samples and 96 animal fecal samples (48 for cattle and 48 for sheep) were collected and examined by using Nested PCR technique followed by DNA sequencing analysis through phylogenetic tree to know the parasite species. The whole infection rate in human was 36.4% (35/96), while in cattle was 22.9% (11/48) and in sheep was 14.5% (7/48) by nested polymerase chain reaction technique. The findings of DNA sequencing based on 18s rRNA gene (654 bp) showed the presence of three Cryptosporidium spp. which namely C. hominis, C. parvum, and C. bovis, all of them have identity 99 %, Cryptosporidium parvum was the most widespread species being detected in 60% (9/15), followed by C. bovis 33.33% (5/15), and C. hominis 6.66% (1/15). This is the first report revealed the molecular sequencing among human, cattle and sheep in Wasit province, Iraq and the role of livestock (cattle, sheep) in zoonotic transmission of the cryptosporidiosis.

Keywords

Cryptosporidium spp, Human, Cattle, Sheep, Sequences Analysis.

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