DOI: 10.14704/nq.2018.16.6.1627

Effect of Sanpian Decoction on CGRP, 5-HT and IL-1β in Peripheral Blood of Migraine Model Rats

Yan Liu, Yonglie Zhao, Jinmin Liu

Abstract


This study investigates the effect of Sanpian Decoction on the calcitonin gene related peptide (CGRP), 5-hydroxytryptamine (5-HT) and interleukin-1β (IL-1β) in peripheral blood of migraine model rats. Methods: A total of 24 healthy SD rats of SPF grade are randomly divided into 4 groups: saline control group, migraine model group and sumatriptan succinate group, Sanpian Decoction group. The experimental migraine animal model is replicated by subcutaneous injection of nitroglycerin on the back of the rats’ neck for 3 weeks. After the second injection of nitroglycerin, drug intervention is given. One week later, the materials are drawn 2 hours after the third injection of nitroglycerin, and the contents of CGRP, 5-HT and IL-1β in peripheral blood of rats in each group are measured by Elisa method. Results: The content of serum CGRP in migraine model group increases, which is different from that in saline control group, the difference is statistically significant (P<0.05); The serum CGRP of migraine model group is compared with that of sumatriptan succinate group and Sanpian Decoction group (P < 0.05); The serum CGRP of sumatriptan succinate group is compared to that of Sanpian Decoction group (P > 0.05); The content of 5-HT in the serum of migraine model group decreases, but there is no statistically significant difference between the groups (P > 0.05); The content of IL-1β in the serum of migraine model group increases, but there is no statistically significant difference between the groups (P > 0.05). Conclusions: Sanpian Decoction can reduce the expression of CGRP in peripheral blood of migraine rats induced by nitroglycerin, increase the content of 5-HT in its peripheral blood, and reduce the content of IL-1β in its peripheral blood, which may also inhibit the transmission of pain information by improving the inflammatory response induced by CGRP.

Keywords


Migraine, Animal Model, Sanpian Decoction, Peripheral Blood, CGRP, 5-HT, IL-1β

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