DOI: 10.14704/nq.2018.16.7.1706

Demonstration of PTZ-Induced Convulsive-Reducing Effect of Butamirate Citrate

Mumin Alper Erdogan, Bilge Piri Cinar, Kubilay Doğan Kılıç, Türker Çavuşoğlu, Gürkan Yiğittürk, Emel Öykü Çetin, Huseyin Avni Balcioglu, Damla Gonenc, Yiğit Uyanikgil, Oytun Erbas


Butamirate has the possible effects on epileptic seizures, which is thought to perform central antitussive effect via medulla oblongata and nucleus tractus solitarius. In our study, these effects have been investigated on electrophysiological and clinical basis. 48 Sprague-Dawley rats were divided randomly into two groups for EEG recordings and behavioral assesment then these two groups divided to four groups: 6 for control, 6 for saline injection, 6 for relatively-low dose butamirate (5 mg/kg) and 6 for relatively-high dose butamirate (10 mg/kg) for each. Evaluation of the behavioral analyses after giving 70 mg/kg pentylenetetrazol (PTZ) first myoclonic jerk time and racine convulsion scales were analyzed, then in different rats for EEG recordings 35 mg/kg PTZ were given and spike percentages were evaluated in same doses of butamirate. In both 5 mg/kg and 10 mg/kg butamirate groups the FMJ onset times were statistically higher then the saline group, similarly both 5 and 10 mg/kg butamirate groups RCS scores were significantly lower than the saline group. In terms of spike percentages, 5 and 10 mg/kg butamirate were significantly lower than the saline group. As a result in our study, we showed that 5 and 10 mg/kg doses of butamirate have anticonvulsant effects on PTZ induced rats.


Experimental epilepsy model; Butamirate citrate; Pentylenetetrazol; Electroencephalogram

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Accorsi-Mendonça D, Almado CE, Bonagamba LG, Castania JA, Moraes DJ, Machado BH. Enhanced firing in NTS induced by short-term sustained hypoxia is modulated by glia-neuron interaction. Journal of Neuroscience 2015; 35(17): 6903-17.

Baulac M, De Boer H, Elger C, Glynn M, Kälviäinen R, Little A, Mifsud J, Perucca E, Pitkänen A, Ryvlin P. Epilepsy priorities in Europe: a report of the ILAE‐IBE epilepsy advocacy Europe task force. Epilepsia 2015; 56(11): 1687-95.

Canning BJ, Chang AB, Bolser DC, Smith JA, Mazzone SB, McGarvey L, Panel CE. Anatomy and neurophysiology of cough: CHEST Guideline and Expert Panel report. Chest 2014; 146(6): 1633-48.

Canning BJ, Mori N. Encoding of the cough reflex in anesthetized guinea pigs. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2010; 300(2): R369-77.

Chien YH, Lin MI, Weng WC, Du JC, Lee WT. Dextromethorphan in the treatment of early myoclonic encephalopathy evolving into migrating partial seizures in infancy. Journal of the Formosan Medical Association 2012; 111(5): 290-94.

Coleridge JC, Coleridge HM. Afferent vagal C fibre innervation of the lungs and airways and its functional significance. Reviews of Physiology, Biochemistry and Pharmacology 1984; 99: 1-110.

Erbaş O, Solmaz V, Aksoy D. Inhibitor effect of dexketoprofen in rat model of pentylenetetrazol-induced seizures. Neurological Research 2015; 37(12): 1096-101.

Ferraro TN, Buono RJ. Polygenic epilepsy. Advances in Neurology 2006; 97: 389-98.

Harden CL. Treatment of sexual disorders in people with epilepsy. Epilepsy & Behavior 2002; 3(5): 38-41.

Jhamandas JH, Harris KH. Excitatory amino acids may mediate nucleus tractus solitarius input to rat parabrachial neurons. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 1992; 263(2): R324-30.

Kang JQ, Macdonald RL. Making sense of nonsense GABAA receptor mutations associated with genetic epilepsies. Trends in Molecular Medicine 2009; 15(9): 430-38.

Klein M, Musacchio JM. High affinity dextromethorphan binding sites in guinea pig brain. Effect of sigma ligands and other agents. Journal of Pharmacology and Experimental Therapeutics 1989; 251(1): 207-15.

Kubin L, Alheid GF, Zuperku EJ, McCrimmon DR. Central pathways of pulmonary and lower airway vagal afferents. Journal of Applied Physiology 2006; 101(2): 618-27.

Lemus M, Montero S, Leal CA, Portilla-de Buen E, Luquin S, Garcia-Estrada J, Melnikov V, de Alvarez-Buylla E. Nitric oxide infused in the solitary tract nucleus blocks brain glucose retention induced by carotid chemoreceptor stimulation. Nitric Oxide 2011; 25(4): 387-95.

McNamara JO, Huang YZ, Leonard AS. Molecular signaling mechanisms underlying epileptogenesis. Science Signaling 2006; 2006(356):re12.

Olsen RW, DeLorey TM, Gordey M, Kang MH. GABA receptor function and epilepsy. Advances in Neurology 1999; 79: 499-510.

Penix LP, Thompson KW, Wasterlain CG. Selective vulnerability to perforant path stimulation: role of NMDA and non-NMDA receptors. Epilepsy Research 1996; 12: 63-73.

Rutecki P. Anatomical, physiological, and theoretical basis for the antiepileptic effect of vagus nerve stimulation. Epilepsia 1990; 31(Suppl 2): S1-6.

Shannon R, Baekey DM, Morris KF, Lindsey BG. Ventrolateral medullary respiratory network and a model of cough motor pattern generation. Journal of Applied Physiology 1998; 84(6): 2020-35.

Shannon R, Baekey DM, Morris KF, Lindsey BG. Functional connectivity among ventrolateral medullary respiratory neurones and responses during fictive cough in the cat. The Journal of Physiology 2000; 525(1): 207-24.

Takaya M, Terry WJ, Naritoku DK. Vagus nerve stimulation induces a sustained anticonvulsant effect. Epilepsia 1996; 37(11):1111-16.

Tan NC, Berkovic SF. The Epilepsy Genetic Association Database (epiGAD): analysis of 165 genetic association studies, 1996-2008. Epilepsia. 2010; 51(4): 686-89.

Uyanıkgil Y, Solmaz V, Çavuşoğlu T, Çınar BP, Çetin EÖ, Sur HY, Erbaş O. Inhibitor effect of paricalcitol in rat model of pentylenetetrazol-induced seizures. Naunyn-Schmiedeberg's Archives of Pharmacology 2016; 389(10): 1117-122.

Walker BR, Easton A, Gale K. Regulation of limbic motor seizures by GABA and glutamate transmission in nucleus tractus solitarius. Epilepsia 1999; 40(8): 1051-57.

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