


Volume 20 No 22 (2022)
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AN ANALYSIS OF THE BACTERIAL POPULATION AND IDENTIFICATION OF HUMAN PATHOGEN PATTERNS IN SOIL SEDIMENTS AND WATER SAMPLES FROM THE PECHIPARAI DAM OF KANYAKUMARI DISTRICT
Sunitha.R, Dr. Deleep Packia Raj.D, Jameer Ahamed. S,
Abstract
The necessary component for all life to survive sustainably on earth and every living depends on water
sources for daily activity, survival, and existence. This potential source is actually becoming contaminated as a
result of industrialization, and modernization of the human population causes microbial discharges and the
excretion of dangerous substances. This condition causes ecological habitations to decline. This inquiry looked at
the bacterial populations in soil sediment samples, water, and pathogenic (SLP and PLS) fish parts accumulation in
the Pechiparai reservoir. The outcome data revealed theabundance of bacterial population about 194 ± 4
CFU/dilution of average in pre-monsoon season of soilsedimentsamplesand water (197± 2 CFU/dilution in average)
over other seasons (On-monsoon and Postmonsoon). Duringmonsoonthe waterbacterialconsistency
wasrefreshinghence the aquaticecosystem is balanced with both micro and macroscopic level organisms. Higher
bacterial inhabitants was recorded in the surface ofNile tilapia (Oreochromisniloticus) and the isolated colonies
werestudied for the identification pathogens (SLSand PLS). Differential media (Macconkey), haemolyticand
biochemical characterization data revealed the presence of Bacillus sps and Staphylococcus sps. Further coliforms
were enumerated in both water and soil sediments on three seasons. Among others, monsoon season exhibited
predominant presence ofcoliforms around 60coloniesinwatersamplesofpre-monsoon season
Keywords
Industrialization, chemical and microbial contaminants,SLS (Staphylococcus like sps), PLS (Pseudomonas like sps), colony forming unit (CFU), Nile tilapia(Oreochromisniloticus), coliforms and Basillussps, Staphylococcussps and monsoon seasons.
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