Toxicology testing is crucial for establishing the characteristics of a test chemical and its hazardous effects(Arome D, 2013). As a result, the OECD test guidelines recommend advice on how to employ techniques for characterization and identifying chemical substances’ hazards. Rosarin, a cinnamyl alcohol glycoside derived from Rhodiola rosea plant, was chosen as test substance for toxicity studies. It is found that the drug Rosarin has anti-inflammatory, neuroprotective, and other therapeutic properties. Different experimental models were subjected to various concentrations of the drug Rosarin for determining the toxicity. The experimental models chosen are zebra fish for determining acute toxicity and to evaluate its histopathological studies, brine shrimp for lethality assay and fenugreek seeds for phytotoxicity. The zebra fish were exposed to different concentrations of drug for 96 hours, and the toxicity levels were determined by analyzing histopathological studies of the drug-treated fish. While brine shrimp eggs were taken and hatched for a period of 24 hours, then the larvae were isolated and percentage of live shrimps was calculated within 24hours. The fenugreek seeds weresown in soil after being treated in various concentrations of the drug for few hours to test for phytotoxicity whose observations need to be recorded once every 24 hours.

Rosarin, Acute toxicity, Histopathology, Lethality assay, Phytotoxicity