Volume 20 No 10 (2022)
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Activation of the Lactoperoxidase system in milk using the enzyme Glucose oxidase(GOX)
Zainab M Ramadhan and AthraaHarjan Mohsen
Abstract
The results of the study showed the ability of the enzyme to inhibit microorganisms, as it amounted to the total number of bacteria on the fifteenth day of concentration (0.05-0.10-0.15)% (1.5×104 – 1.3×104 – 0.9×104 ) CFU/ml on the respectively, while the total number of bacteria for the control treatment was (1.8×104 ) CFU/ml. And there was no growth of coliform bacteria on the first day of all treatments, and the growth began for the control sample on the fifth day, as it amounted to (0.8×104 ) CFU/ml, while there was no growth in the rest of the treatments, and on the 15th day it amounted to ( 2.8 x 104 ) CFU/ml and it reached (0.6 x 104 ) CFU/ml for the concentration (0.05)%, while there was no growth for the concentrations (0.10-0.15)%. The percentage of the total number of yeasts and molds on the fifteenth day in relation to the concentrations (0.05 - 0.10 - 0.15) % (0.7 x 104 - 0.6 x 104 - 0.2 x 104 ) CFU/ml, respectively. The results indicated a significant increase in the acidity of the control treatment compared to the treatments to which the enzyme was added, and the treatment with concentration (0.15) was superior, as it remained acidity until the fifteenth day, and the results indicated a significant decrease in the pH value of the control treatment if it went outside the acceptable limits. On the tenth day, while the treatments to which the enzyme was added did not go beyond the acceptable limits, and the pH value remained maintained throughout the 15 days of storage
Keywords
Lactoperoxidase, milk, bacteria count, Total Titritable Acidity
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