The main objective of present work was to study pharmacognostical and phytochemical evaluation of Operculina turpethum linn extract for the treatment of hyperuricemia. The plant Operculina turpethum linn (Convolvulaceae), has been traditionally used for the treatment of various diseases and in ayurvedic literature is described as sarvanubhoothi, Trivit. The plant roots were extracted with different solvents including pet ether, ethanol and aqueous in that ethanol extract of plant subjected to phytochemical and pharmacognostical study. Enzyme assay was done by using bovine milk xanthine oxidase (XO). The XO inhibitory activity in vitro was performed by using different doses of root extract and the degree of XO inhibition was expressed as IC50.The antihyperuricemic activity of Operculina turpethum were tested in the potassium oxonate-induced hyperuricemic rats for seven consecutive days of oral treatment of 100 mg and 200 mg/kg doses. The results of study show that total flavonoid and phenolic content was estimated. Physico-chemical properties revealed total ash (5.00w/w), acid insoluble ash (1.32), water soluble ash (1.05w/w), water soluble extractive (15.60w/w), petroleum ether soluble extractive (2.00w/w), ethanol soluble extractive (19.20w/w), loss on drying (3.12w/w). The inorganic metal analysis showed the absence of toxic metals and quantification of heavy metals shows the presence of elements within the limit. Fluorescence analysis exhibited considerable variation and preliminary phytochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoids, glycoside, phenol, steroid, tannins and saponins. TLC studies showed that the ethanolic extract showed a single spot in both glycoside and flavonoid solvent system. The ethanolic extract of Operculina turpethum has modrate activity of XO inhibition with IC50=92.51 ug/ml. In addition, these results showed that all doses of Operculina turpethum root extracts were able to significant reduce serum uric acid levels in the hyperuricemic rats. So, present study confirms the antihyperuricemic activity of operculina turpethum root extracts.

Operculina turpethum linn, Ethanolic Extract, total flavonoid, phenolic content, xanthine oxidase, antihyperuricemic,