Green production of silver nanoparticles (AgNPs) utilizing plant extracts is now generally recognized due to its low cost, environmental friendliness, and low toxicity. In the present study, an attempt has been made to synthesize AgNPs by using combination of two different plant extracts namely Azadirachta indica (Neem) and Aloe barbadensis (Aleo Vera). A simple green synthesis method was used for silver nanoparticles production using aqueous leaf extracts of Azadirachta indica and Aloe barbadensisin the combined form. Various characterization techniques were used to characterize the synthesized nanoparticles like UV-Visible spectrophotometer, FTIR, SEM and XRD. The In-vitro anti-inflammatory were done by Inhibition of albumin denaturation, In-vitro antioxidant activity was done by ABTS free radical scavenging assay , DPPH assay and Potassium Ferricyanide Reducing Power assay method then In-vitro antimicrobial studies against Bacillus cereus, Actinomycetes Israeli, Proteus vulgaris, Streptococcus pyogenes, Corynebacterium diphtheria, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus were examinedat various concentrations (50,100,250,500µg). The results indicated that biosynthesized Silver nanoparticles (AgNPs) using aqueous leaf extracts of Azadirachta indica and Aloe barbadensis in combination work as stabilizing agents. Our research demonstrated the anti-inflammatory, antimicrobial and antioxidant efficacy of phytomediated silver ions in vitro.

Combined leaf extract, Green synthesis, Aloe barbadensis, Azadirachta indica, Silver nanoparticles, anti-inflammatory, antioxidant activity and antimicrobial activity.