Volume 20 No 6 (2022)
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Anti-Microbial Activity and Phytochemical Screening of Citrus Medica Leaf Extract
Tankadhar Mishra , Bibhuti Bhusan Panigrahi , Manoj Kumar Pani
Abstract
Citrus medica is an important medicinal plant of the family Rutaceae Qualitative and quantitative screening were performed for evaluation bioactive
constituents in Citrus medica fruit. The antibacterial activity of the ethyl acetate and ethanol 80% Leaf extract of Citrus medica was assessed by agar
well diffusion assay, minimum inhibitory concentration (MIC) value determination by 96 well serial dilution against five gram positive and two gram
negative bacteria, while minimum bactericidal concentration (MBC) evaluated by plate cultures.
Keywords
Citrus Medica, Escherichia coli, Agar well diffusion method.
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