The production of AgNPs through the “Green route” process has gained the consideration of many researchers and numerous studies have been carried out in current years because of its ease of use and environmental approachability. In the present investigation, apple cider vinegar has been utilized for the production of AgNPs for its anti-cancer activity determination. Characterization techniques such as FTIR, Ultraviolet-Visible spectroscopy, SEM and HPLC have been utilized to confirm the biosynthesis of AgNPs. For the anti-cancer activity determination, HeLa cell lines were used for the MTT assay, DAPI and PI staining and ROS generation assay. During the MTT assay, the percent cell viability of HeLa cancer cell line reduced in a dosage dependant method upto 50.66% at 100 microgram/millilitre conc. when compared to a concentration of 10µg/ml (90.50%) with an inhibition concentration (IC50) at 90µg/ml. The cytotoxicity effects of biosynthesized AgNPs were confirmed by using DAPI and PI staining method. During the PI staining method, PI positive cells were present in negligible amount in the control whereas the number of positive cells progressively increased with rise in AgNPs concentration showing maximum number of PI positive cells at a conc. of 80 microgram/millilitre. The results of DAPI staining revealed that with the increase in AgNPs concentration the permeability of HeLa cells to DAPI staining also increased as compared to control. The increase in cell permeability confirmed the significant apoptotic efficiency of AgNPs in HeLa cells in a dosage dependent way. Also, in the results of the intracellular ROS analysis, a dose-dependent rise in the intra-cellular Reactive Oxygen Species generation was observed when equated with control which indicates early apoptosis induction of HeLa cells as a result of increase in oxidative stress. From the data obtained, it can be concluded that the apple cider vinegar-AgNPs have a significant anticancerous potential and can be used in future in biomedical and pharmacological industrie

Silver nanoparticle, Apple cider vinegar, HeLa cell line culture, MTT assay, Apoptosis, ROS generation, Anti-cancer activity, DAPI and PI staining