Volume 20 No 22 (2022)
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Biological Synthesis and Characterization of Silver Nanoparticles of Camelia Sinensis and Evaluation of Its Biological Activity
B. Swapna, K.B. Chandrasekhar, D. Madhuri, G. Sumathi
Green tea leaf aqueous extracts of varying volumes (5ml, 10ml, 15ml, 20ml, 25ml) are outlined for the green synthesis of silver nanoparticles using a 1Mm silver nitrate solution. UV-visible absorption spectroscopy, FTIR, SEM, and HPLC are utilized to evaluate synthesized nanoparticles. The synthesis was predicated on the phenomenon of surface plasmon resonance, as determined by UV- spectra at 200-400 nm, which confirmed the presence of silver nanoparticles. The Fourier-transform infrared (FTIR) spectrum confirms the presence of Amine, Alcohol, Aldehyde, Aromatic, C-H bending, and C-O bending functional groups as concentrations of green tea leaf extracts increase. The Scanning Electron Microscopy (SEM) analysis reveals a helical structure of silver nanoparticles ranging in size from 5 to 100 nm, which exhibits good antioxidant and antimicrobial properties against pathogens such as E. coli and Staphylococcus aureus, as well as good antimicrobial activity.
Green tea leaf aqueous extract, Silver Nanoparticles, UV-VIS, FTIR, SEM, HPLC, Antioxidant activity and antimicrobial activity.
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