Nowadays, Plants are assessed for their traditional therapeutic properties, as a replacement or a superior option for chemical-based medications. In the similar context, the present work was designed for the Qualitative and quantitative evaluation of phytochemicals, anti-microbial, and antioxidant activities of the leaf extracts of Hibiscus callyphylus pertaining to its pharmacological utilisation. Qualitative preliminary phytochemical analysis depicted that alkaloids, phenols, carbohydrates, flavonoids, amino acids, tannins and terpenoids were present in these extracts. The maximum antioxidant activity was shown by methanolic extracts pertaining to the lowest IC50 values followed by aqueous, chloroform and petroleum ether extracts. Total phenolic and flavonoid content was also highest in methanolic solvent. HPLC analysis revealed that only two flavonoids viz. Rutin and Naringin and the representative phenols viz. Gallic acid, Caffeic acid, Ferulic acid, Cinnamic acid and Pyrogallol were present in H. callyphylusleaf extracts. The results showed that the tested plant extract possessed Naringin as a major flavonoid and pyrogallol as a major phenolic constituent.From the anti bacterial studies it can be concluded that inhibition activity of the extracts significantly depend upon the solvent used and microbial strain tested for susceptibility. Methanolic extract were found to be more effective against all the tested bacterial strains. The present work provides brilliant basic information regarding the phenols and flavonoids compounds present in the leaves of H. Callyphylus having excellent antioxidant and antimicrobial potential, which will play an important role for further research to isolate these phytochemicals for various purposes.

Anti-bacterial activity; antioxidant activity; DPPH assay; flavonoids; phenols; phytoconstituent