Advances in next-generation sequencing (NGS) RNA-Seq technology is making it possible to reveal differentiated thyroid cancer (DTC) genotype. Little is known about the genes that are active in this pathology. The expression profile of CDT stem cells in the Arequipa population is described. 11 patients with CDT undergoing thyroidectomy between 2019-2020 from Hospital Nacional Carlos Alberto Seguín Escobedo EsSalud Arequipa were enrolled, and stem cells were isolated from biopsies. Total RNA was isolated from the stem cell cultures and subjected to massive sequencing by RNA-Seq using the Illumina protocol. The most frequent histopathological variants were papillary (81.82%) and follicular (18.18%). Bioinformatic analysis of the massive sequencing (RNA Seq), using IGV software and the GRCh38.p13 genome, showed a prevalence of mutations in BRAF, TERT and P53 genes in all patients. The top 30 genes with the highest frequency of expression were: PCB2, LRMDA, WWOX, RPS29, RBFOX1, FHIT, NTM, RAD51B, PRKN2, MACROD2, ASIC2, TBC1D5, GPC5, EXOC4, IMMP2L, CAMTA1, CDH13, SMYD3, ANKS1B, DAB1, DLG2, FRMDA4A, KCNIP4, CTNNA2, AGBL4, PRKCE, SYN3, ARHGAP1 5, PACRG, C9orf92. This study reports for the first time five new mutations for the BRAF gene and 18 new mutations for the TERT gene

Thyroid cancer, RNA-Seq. Stem cells