Volume 20 No 9 (2022)
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Comparison Of Ultrasound-Assisted And Conventional Solvent Extraction Techniques For Characterization Of Phenolic And Flavonoid Compounds From fresh Leaves Of Alpinia Mutica
Shankaraiah Pulipaka, Ashish Suttee, M. Ravi Kumar , Ramesh Kasarla , Swamy Kasarla
Abstract
Since a few years ago, the traditional methods, such as the Soxhlet and reflux apparatushave been used most frequently to extract plant material. However, these procedures take a long time and a lot of solvent. Today, the innovative extraction method is employed to extract plant material in order to solve this issue. The ultrasonicassisted extraction process is superior to the traditional method in that it uses less solvent, takes less time, and produces more bioactive phytoconstituents. Alpinia mutica (A.M.) is an Indian herb that is a member of the Zingiberaceae family and is used as a traditional treatment. The Zingiberaceaefamily's largest genus, Alpinia, contains over 230 herbs that are widely used throughout Asia's distinctive and sub-peculiar regions. In southern India, the plant is said to contain 9 different species of plants. Alpinia mutica is a perennial herb that grows in Malaya and the Kingdom of Thailand. It produces horizontal, subterranean stemmed, scented plants. Although cultivation has undergone a few adjustments, the sorted types are scattered throughout northern Malaysia. The northern region of the Malayan foreland is home to a variety of species, despite the fact that there are some agricultural sources that provide alternatives to A.M. Locals utilise these plants to alleviate stomach gas issues, and the fruits are used to relieve edoema. Alpinia mutica leaves were extracted using both traditional and ultrasonic methods using a variety of solvents, including petroleum ether, ethyl acetate, methanol, hydroalcohol, and aqueous solvents. When compared to traditional approaches, the results show that the ultrasonic-assisted extraction procedure yields a high yield of phytoconstituent.In order to determine the overall phenolic and flavonoid content of the extracts produced by both processes, further analysis was performed on them. According to the research, the phenolic and flavonoid content of the extract has been noticeably boosted by the use of ultrasonic-assisted extraction procedures. Additionally, the ultrasonic-assisted extraction method for plant extraction proved to be a quick and effective solution.
Keywords
Alpinia mutica, Ultrasonic-assisted extraction technique, Optimization
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