Comparison of Conventional Virus Isolation Technique With RTPCR For Enterovirus Detection In Stool Specimens From Patients of Acute Flaccid Paralysis

Volume 20 No 9 (2022)

Jasmeet Singh, Harjeet Singh Maan, Dharamveer Singh, Nikky Nyari Srivastava, Atul Garg, Shruti Shukla, Rachna Chaturvedi

DOI: 10.14704/nq.2022.20.9.NQ440104

Abstract

Every year Enteroviruses cause different diseases and infect millions of people. Cell culture is conventional method of choice for the detection of enteroviruses (EVs) in Acute Flaccid Paralysis (AFP) cases. In this study, we compared cell culture and polymerase chain reaction (PCR) methods for the detection of EVs in AFP suspected children below 15 years of age. A total of 789 stool samples were cultured in Rhabdomyosarcoma (RD) cell line. The specimens were tested for 5´ UTR by Pan EV Real-time PCR and VP1 PCR. A total of 109 were found positive by cell culture, 163 by RT-PCR and 152 by VP1 PCR, so increasing the percentage of identification from 13.81% to 20.65%. PCR was recorded higher sensitivity than the cell culture method. In conclusion, molecular methods were found to be useful for rapid and specific detection of EVs with higher sensitivity compared with the conventional cell culture method will make more confident for the detection of EVs

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