Because of its importance in numerous physiological process , SeNPs research has gotten greater interest in recent years. In comparison to selenium, selenium nanoparticles have a high level of absorption in nutritional supplements . SeNP has been synthesized using a variety of chemical and biological techniques. Because of its anti-oxidant, anti-bacterial, anti-diabetic, and anti-cancer properties, selenium nanoparticles have biological and pharmacological applications . This article reviews biosynthesis of SeNPs using Na2SeO3 and Bacillus clausii under aerobic conditions .The structural and morphological properties of SeNPs were determined by UV_Vis spectroscopy and the absorption peak was observed at ( 260 nm) wavelength , in SEM size range between (37.58 – 75.16 nm) , the average diameter of SeNPs was 19.28 in AFM . Results showed that no significant cytotoxic effect of SeNPs against PC3 cells at concentrations (25 and 50 µg/mL) . Nevertheless, SeNPs at (100, 200 and 400 µg/mL) exhibited a dose_dependent_decrease in PC3 cell viability with maximum inhibition rate of 51.27±2.77% of PC3 cells at 400 µg/mL

SeNPs , Bacillus clausii , UV- Vis spectroscopy , SEM , AFM , PC3 and WRL 68