Development and validation of RP-HPLC method for simultaneous estimation of Amitriptyline Hydrochloride and Propranolol Hydrochloride in pharmaceutical dosage form.

Volume 20 No 13 (2022)

Akanksha Gajmal, Ganesh S Andhale, Vishnu S Neharkar3, Kishori Hol Sapana M Nagare, Dr. Ganesh S. Andhale

DOI: 10.14704/nq.2022.20.13.NQ88174

Abstract

Reverse phase high performance liquid chromatography method has been developed and validated for simultaneous estimation of Amitriptyline Hydrochloride and Propranolol Hydrochloride in dosage form. This method uses C18 agilent column with 4.6 x 250mm length and 5μm particle size of packing material. Mobile phase is methanol: water pH 6 with TEA (70:30) with 1ml/min flow rate and 20μl volume injected. UV detection was carried out at 216 nm and the column temperature is 250C. The retention time of Amitriptyline Hydrochloride was 3.882 min. and 6.384 min of Propranolol Hydrochloride. The method is validated and calibration curve observed was linear in the concentration range of 5-25μg/ml for Amitriptyline Hydrochloride and 20-100μg/ml for Propranolol Hydrochloride. The method is validated for linearity, accuracy, precision, limit of detection and quantification, ruggedness and robustness

Keywords

Amitriptyline Hydrochloride, Propranolol Hydrochloride, RP-HPLC, UV Detection

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