The anti-hyperlipidemic effect of Polyherbal formulation (PHF) was studies in Triton WR-1339 induced hyperlipidemic in rat models. Here, Acute hyperlipidemia was induced by administration of single dose of Triton WR-1339 (400 mg/kg,i.p). Triton WR-1339 induced animals significantly increased serum Total Cholesterol, Triglycerides, Low Density Lipoprotein Cholesterol (LDL-C), Very Low Density Lipoprotein Cholesterol (VLDL-C) , Treatment with PHF (200 and 400 mg/kg, p.o ) significantly reduced the hyperlipidemia i.e., decreased levels of serum Total Cholesterol, Triglycerides, Low Density Lipoprotein Cholesterol (LDL-C), Very Low Density Lipoprotein Cholesterol (VLDL-C) , an increase of serum High Density Lipoprotein Cholesterol (HDL-C) when compared to vehicle control and standard Atorvastatin (10 mg/kg). Histological study on heart showed amarked regression compare to control. The results demonstrated that PHF possessed significant antihyperlipidemic activity

Lipoprotein, PHF, Triton WR-1339