The aim of the present study was to investigate the effect of three techniques for oocytes collection on retrieval and quality of oocytes collected from local buffalo ovaries. 240 ovaries were collected from 120 non-pregnant buffaloes with 3-8 years old, slaughtered in the slaughterhouses in Babil Governorate for the period from September 2020 to February 2021. The oocytes were collected using ovarian slicing , ovarian puncture and follicle aspiration techniques, then the oocytes were classified according to the number of ovarian cumulus cells layers and cytoplasm homogeneity into three groups, first: good oocytes surrounded by more than three layers of cumulus cells and homogenous cytoplasm, second: medium oocytes surrounded by fewer than three layers of cumulus cells and homogenous cytoplasm, and third: poor oocytes denuded of cumulus cells with heterogeneous cytoplasm. The results indicated a significant increase (P <0.05) for the total number of retrieved oocytes and the average number of oocytes per ovary using the ovarian slicing technique compared with the ovary puncture and aspiration techniques. Results of this study was also recorded a significant increase (P <0.05) for the total number of oocytes, average number of oocytes per ovary, and the percentage of good oocytes obtained by ovarian dissection compared with ovarian puncture and follicle aspiration techniques. It could be concluded from this study that the ovarian slicing technique was the appropriate and good technique for oocytes retrieving in large numbers, with good morphology and quality.

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