This study aimed to detect single nucleotide polymorphisms (SNPs) of the CAPN1 gene in cattle using 139 animals of the southern and crossbreed cattle breeds, 73 and 66 calves of the two breeds, respectively, and to know the relationship of these SNPs in meat characteristics specific to tenderness. Blood and meat samples were collected and tested. The technique of polymerase chain reaction (PCR) was used, and the technique of DNA sequencing was used to detect the genetic structures of the gene. The frequency of the genotypes of the CAPN1 gene (GG), CG and (CC) was 85.00, 6.80 and 8.20%, respectively, in the southern cattle breed, and 56.00, 32.00 and 12.00% in the breed of crossed cows at site 316, and the genotypes AG and GG were (68.00 and 32.00)% for the southern and (26.00 and 74.00)% for the racket, respectively, at site 530. As for the allelic frequency of the alleles, the percentages ranged from low to high. Significant differences appeared (P<0.05)) according to the genotypes of the studied traits, as the two structures outperformed The genotypes GG and AG in most of the traits and CG in some traits in the current study, which included the concentration of myoglobin pigment, the amount of metamyoglobin, the fiber breakdown index, total and soluble collagen and myofibrillar proteins.

polymorphism , CAPN, tenderness , Crosses , cattle