This study was conducted to find out the potential efficiency of Ozone treatment in detoxification of mycotoxins Nivalenol (NIV) and Fumonisine B2 (FB2) and Deoxynivalenol (DON) associated with Triticum aestivum, grains, as well as their effect on wheat seeds quality including, essential amino acids (EAA), free fatty acids (FFA), and the protein (Gluten). For this purpose, ozone gas was applied at a concentration of 2000 mg / hour at 40 ° C at a period of 24 hours exposure. The results showed that ozone gas was effective in reducing NIV and DON. The reduction percentages were 28.8 and 60.7%, respectively, while the reduction rate of FB2 was 100%. Ozone treatment had a significant effect on chemical components of wheat grains unevenly; the results showed EAA was divided into two groups, the first group revealed an increase in the levels of EAA including: Aspartic, Glycine, Cystine and Methionine, whereas the second group showed a decrease EAA such as Glutamic, Serine, Histidine, Threonine, Arginine, Alanine, Tyrosine, Valine, Phenylalanine, Isolieucine, and Lysine. However, Leucine was found undetected after the treatment. Fatty acids profile showed similar pattern of results; two distinct groups were examined; the first one with an increased level of FFA including Oleic and Linolenic; another FFA group included a decreased level of Palmitic, Palmitoleic and Linoleic. Finally, there is no significant difference in Gluten protein levels in the treated wheat grains with Ozone compared to the control treatment, with average of 2.1 and 2.17 g, respectively

Detoxification, Mycotoxin, Ozone, Triticum Aestivum, Wheat Quality