The aim of this study was conducted to elimination biofilm production from K. pneumonia by Sr90 radiosources inorder to removal biofilm that contained with K. pneumonia infection by beta ray. Study design and study populations of 100 isolates of K. pneumonia that identified by Vitek2GN, results of identification for all isolates approve are K. pneumonia. Study production of biofilm achieved by Congo red agar test, positive results by formation black colony on Congo red agar and negative results with red colony on Congo red agar. Influence Strontium Sr90 on K. pneumonia with dose D*(1hr.) 0.73251*10-2 msV; D*(2hr.) 1.46502*10-2 msV; D*(3hr.) 2.19753*10-2 msV; D*(4hr.)2.93004*10-2 msV in different hours percentage of killing for this bacteria reach to 90% with control 300 colony,elimination of biofilm production from this bacteria by all doses in different time with formation pink color of colony compared with control formation black colony on Congo red agar, this results of affect by Strontium cause lost production biofilm by turn off into pink color when exposed to different doses of beta rays emitted from Strontium compared with control with black color

Radiation, Bacteria and Biofilm