Fungal infection of skin is now-a-days one of the common dermatological problem. The present work aimed to prepare and evaluate the liposomal formulation of an anti-fungal drug Bifonazole. Total nine batches of Bifonazoleloaded liposome was prepared by thin film method using varying ratio of cholesterol, soya lecithin and stearyl amine. The amount of soya lecithin was kept constant in all batches. Stearyl amine was used as charge imparting agent. The effect of formulation variable on entrapment efficiency, particle size, surface charge and drug release behaviour was studied. The optical microscopy and SEM study demonstrated that the particles had almostspherical and uniform shapes and did not aggregate to each other. Encapsulation efficiency of Bifonazoleloaded liposomes was determined by centrifugation method. The inclusion ofpositively charged surfactant such as stearyl amine significantly increases the entrapment efficiency of Bifonazole into the liposomes. The LF8 formulation showed the highest drug-loading capacity of 94.34%±3.03 when compared with the other formulations. The cumulative amount of drug release from Bifonazole liposome was 57.25%-93.21%, after 24 hours. The release of Bifonazole from liposomes changed with phospholipid and cholesterol ratio (LF1-LF6). Drug release delayed as molar ratio of soya lecithin increased in the formulation (p < 0.05). This might be due to the interaction of Bifonazole with the large surface of lipid bilayer membrane of liposome and soya lecithin layer thickness and fluidity of membrane. In conclusion, stable Bifonazole loaded liposomal formulation was successfully formulated for the topical administration

Bifonazole, Liposome, soya lecithin and fungal infection