Anxiety is a mental disorder that alters a person's way of life and places a heavy burden on the patient's ability to afford therapy. You can start to perspire, become agitated and anxious, and have fast heartbeat.The object of present investigation was in order to ascertain whether Cyanthillium cinereum (L.) H. Rob's methanolic extract had any anti-anxiety effects on mice. The physiochemical, phytochemical, and anti-oxidant activity of C. cinereum was examined. Different significant parameters, including ash value, moisture content, fiber content, and extractive values for extract with methanol, extract with ethyl acetate, extract with chloroform, and extract with water, were reported by physiochemical assessment. The phytochemical investigation revealedthat C. cinereum contain alkaloid, saponin, flavonoid, terpenoids and phenol. The outcomedemonstrated that methanolic extract the better solvent for Enhancement of methanolic extraction by FTIR and LCMS analysis. The anxiety activity of methanolic extract was determined and result of antianxiety activity recorded that significantly(p< 0.0001) increase the effect of diazepam 2mg/kg and plant extract 400mg/kg as comparedwith plant extract 100mg/kg and 200mg/kg compared data with control group. Thus, thestudy proved that methanolic extract of C. cinereumhad good potential as anti-oxidant,antianxiety activity and thus this plant can be utilized as natural source for CNS activity.

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