Volume 20 No 10 (2022)
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Examination of the Toxicity of Gold Nanoparticles Manufactured by Green Methods from the Leaves of Lantana Camara Plant on Some Cell Lines
Bushra Ryadh Mohi , Dr. Ismail Abbas Jdyea , Dr. Ahmed Fadil neima
Abstract
Gold nanoparticles were synthesized from the hot ethanolic extract not from the leaves of the enamel dendritic plant and their properties were studied by using the SEM scanning microscope to determine the surface shape and the structural size of the biosynthesized gold nanoparticles from the enamel dendritic plant. The results show that the nanoparticles ranged between 40-80 nm, the working force microscope was used as follows: Shapes, topography, roughness and bumps of gold nanoparticles, and the average roughness size of the nanoparticles 11.5 nm. Angle 51, angle 66 and angle 73 nm There is an X-ray diffraction database ICDD file. 220-220-311 UV-visible spectrometer was used to detect gold particles, which showed that the absorption peak of gold particles was at wavelength 400-480 nm and the highest absorption was at wavelength 480 nm. The particles have cytotoxicity against cancerous tumors through their effect on the cells of the liver cancer cell line (HEPG2) and compared it with the normal cells (WRL68) and the degree of its effect depends on the concentration. Dendritic enamel. The inhibition values were (37.35 and 3.59) and (400 and 25) µg/ml respectively for HEPG2 cell line. As for the normal cell line WRL68, it was as follows (26.81 and 4.2) for the same concentrations, and the IC50 concentration for cancer cells was (237.1) µg/ml and for the normal cell line (110.0) µg/ml when compared with the results of the alcoholic extract, which were on the cell line as follows (51.08) and 3.25 for the concentrations (400 and 25) µg/ml, respectively, and the inhibitory value of the extract on the normal cell line was (48.42 and 4.71) and for the same concentrations as above, it reached IC50 µg/ml for cancer cells 146.7 and for normal cells (150.0) µg/ml.
Keywords
Toxicity, Nanoparticles, Green Methods, Lantana Camara
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