Bacteria can develop resistance to antibiotics through several mechanisms. In this study, we focus on one of the mechanisms based on biofilm growth. Biofilms are organized communities of micro organisms buried firmly on extra polymeric matrix attached to the living surface. The matrix which is formed surrounding the bacteria makes them tolerant to harsh environment conditions as well as resistant to an tibacterial treatment. The development of biofilms in clinical or hospital setting is the major reason for several infections. They have negative impact when they are formed on medical devices as it is difficult to eradicate it using disinfectants or antib iotics available. Several in vitro models have been implemented in the last decade to evaluate antimicrobial activity on bacterial biofilms. T here is lack of simple economical, and reliable static biofilm models to study biofilms In this scenario, consid ering the importance of this research area, we planned to design laboratory scale working models to study biofilms. We us ed Escherichia Coli as a model organism.

Biofilms, Ant ibiotic resistance, I nfection, Escherichia coli static model