Oxidative distress is a key factor for both acute and chronic illnessesand neuro degenerative disorders. The pathophysiology of various illnesses has been related to free radicals and other Reactive Oxygen Species (ROS).Antioxidant compounds such as phenols, flavonoids and tannins scavenge free radicals and thus delay or prevent oxidative damage to the targeted tissue. The combination of two or more drugs or plants is referred to as Polyherbal Formulations (PHF). A Polyherbal formulation increases the therapeutic effect and shows synergism. It also reduces the adverse effects by reducing the concentrations of single herbs.The objective of the present study is to quantify Total Flavonoid Content (TFC), Total Phenolic Content (TPC), Total Tannin Content (TTC) and to evaluatean in-vitroantioxidant activity of hydroalcoholic extract of Cassia auriculata leaf, Centella asiatica leaf, Zingiber officinale rhizome and its Polyherbal Formulation (PHF)by three different methods such as Ferric Reducing Antioxidant Potency (FRAP), DPPH and ABTS radical scavenging assay. Quantitative estimation of TPC, TFC and TTC of individual extracts and Polyherbal Formulation showed positive results. TPC and TTC are higher in PHF as compared to individual extract except for Cassia auriculata. TFCis higher in Zingiber officinale followed by Cassia auriculata, PHF and Centella asiatica. All individual extracts and PHF exhibited significant antioxidant activity in all 3 methods. The PHF showed higher radical scavenging activity as compared to individual extracts.This might be due to the synergistic effect of its bioactive phytoconstituents. Hence, the outcome of this study proved the beneficial effect of PHF over individual extracts

Antioxidants,Cassia auriculata, Centella asiatica,Quantitative estimation,Zingiber officinale