In situ gel formulations are those formulations that rapidly undergo a sol to gel system after the administration in the eye and significantly improve the bioavailability and pre- corneal residence time. Thiswork describes the formulation and evaluation of in situ ciprofloxacin hydrochloride in situ gel that showed phase transition on the corneal surface. Ciprofloxacin hydrochloride is widely used as a broadspectrum antibiotic in the treatment of corneal ulcers or ocular infections. In this formulation, the chitosan is chosen as a polymer that acts as an ophthalmic gel-forming agent and this mucoadhesive polymer undergoes instantaneous gel formation by interacting with divalent cation (Ca+2) of lachrymal fluid (pH 7.4). Hydroxy Propyl Methyl Cellulose (HPMC) is used as a copolymer and enhances the viscosity hence providing sustained drug delivery. The formulationsdeveloped were evaluated for the analysis of different physical-chemical parameters such as clarity, pH measurement, gelling capacity, drug content, rheological study, and in vitro drug release

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