Green synthesis of silver nanoparticles using the aqueous leaf extract of Aerva lanata and Momordica charantia were screened for antimicrobial activity by chick chorioallantoic membrane assay (CAM) (inovo method). Green synthesis of silver nanoparticles using the aqueous leaf extract of Aerva lanata and Momordica charantia were screened for antimicrobial activity using the cup plate method against gram positive and gram-negativereference organisms (Bacillus Subtilis and Escherichia coli). The extracts were incubated with AgNO3 solution showed gradual change in colour to yellowish brown, with intensity increasing during the period of incubation. The nanoparticles were characterized for particle size, zeta potential and Transmission Electron Microscope (TEM). The silver nanoparticles prepared were found to be spherical in shape with nano size and stable. The biosynthesized silver nanoparticles (AgNPs) showed good antibacterial activity against clinical strains of two bacteria. The antiangiogenic activity of extract was screened by chick chorioallantoic membrane assay (CAM) (in-ovo method). In CAM assay, number of branches and angiogenic-score were evaluated and significant results were observed at 10-4M. Green synthesis of silver nanoparticles using the aqueous leaf extract of Aerva lanata and Momordica charantia were screened for antimicrobial activity and showed good antibacterial activity against these two clinically active strains. The nano extracts also showed significant antiangiogenic property in the CAM assay.

Aerva lanata, Momordica charantia, silver nanoparticles, antimicrobial activity, antiangiogenic activity.