This research was performed to study the selected medicinal plants Cassia auriculata, Gymnema sylvestre, and Tinospora cordifolia against invitro and in silico antidiabetic activity. These plants were collected and extracted with different solvents. The alcohol extracts of these plants were used in the in vitro study. The major active constituents were identified from the selected medicinal plants, and 3D structures of the active constituents were retrieved from pubchem chemical databases or chemsketch software. Docking studies were carried out on selected phytochemicals against alpha amylase using Molegro virtual docker. Inhibition of alpha-amylase enzyme in an in vitro study was performed by using the standard protocol against plant extracts. In-silico docking results show the ability of the phytoconstituents to bind with the targets, as measured by the moldock score. The moldock score was used as the parameter for analyzing the docking results, by which the phytoconstituents were ranked. The ligand possessing the highest molecular dock score showed a strong affinity towards its target. Ranking based on moldock score and H-bonding of phytoconstituents from Cassia auriculata, Gymnema sylvestre, and Tinospora cordifolia on alpha amylase (PDB ID: 3OLD). In-vitro antidiabetic activity was performed by the alpha-amylase inhibition method. Inhibition of the alpha amylase enzyme would delay the degradation of carbohydrates, which would in turn cause a decrease in the absorption of glucose and a reduction in postprandial blood glucose levels. Based upon the experimental evidences, it was found that Gymnema sylvestre (92%) possesses higher antidiabetic activity when compared to Cassia auriculata (79%) and Tinospora cordifolia (64%). The in-silico and in-vitro studies reveal that Gymnema sylvestre possesses the potential diabetic control activity against the alpha amylase.

in-silico; in-vitro; anti-diabetic; Cassia auriculata; Gymnema sylvestre; Tinospora cordifolia