


Volume 20 No 13 (2022)
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In Vitro Hypoglycemic Effects Of Black Seed (Nigella Sativa)
Fahad Saad Alhodieb
Abstract
Type-2 diabetes mellitus (T2DM) is one of the major noncommunicable disease worldwide wherein, inhibition of
intestinal glucose absorption and transport from the luminal side to the circulation is considered a critical pathway
for its prevention and treatment. The current study thus explored the antidiabetic potential of black seed using
various in vitro methods, such glucose adsorption, glucose diffusion retardation, carbohydrate hydrolyzing enzyme
inhibition, glucose transport enhancement across the yeast cells. Results indicated that black seed powder (BSP)
effectively adsorbed significant amounts of glucose, inhibited its diffusion across dialysis membrane both in the
presence and absence of α-amylase. BSP exhibited higher glucose dialysis retardation index (GDRI) compared to
wheat bran. The aqueous extract of black seed inhibited α-glucosidase in a dose dependent manner and also
promoted glucose transport across yeast cell membrane in a dose dependent manner. It is concluded that the
observed hypoglycemic effect of black seed is due to the aforementioned mechanisms in addition to previously
suggested mechanism of increasing insulin secretion by pancreatic cells.
Keywords
Antidiabetic, black seed, glucose transport, in vitro, starch digestion
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