Volume 20 No 13 (2022)
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In Vitro Hypoglycemic Effects Of Black Seed (Nigella Sativa)
Fahad Saad Alhodieb
Abstract
Type-2 diabetes mellitus (T2DM) is one of the major noncommunicable disease worldwide wherein, inhibition of intestinal glucose absorption and transport from the luminal side to the circulation is considered a critical pathway for its prevention and treatment. The current study thus explored the antidiabetic potential of black seed using various in vitro methods, such glucose adsorption, glucose diffusion retardation, carbohydrate hydrolyzing enzyme inhibition, glucose transport enhancement across the yeast cells. Results indicated that black seed powder (BSP) effectively adsorbed significant amounts of glucose, inhibited its diffusion across dialysis membrane both in the presence and absence of α-amylase. BSP exhibited higher glucose dialysis retardation index (GDRI) compared to wheat bran. The aqueous extract of black seed inhibited α-glucosidase in a dose dependent manner and also promoted glucose transport across yeast cell membrane in a dose dependent manner. It is concluded that the observed hypoglycemic effect of black seed is due to the aforementioned mechanisms in addition to previously suggested mechanism of increasing insulin secretion by pancreatic cells.
Keywords
Antidiabetic, black seed, glucose transport, in vitro, starch digestion
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