Volume 20 No 9 (2022)
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Influence of pH and concentration, on the degradation of Sodium Cyanide by Pseudomonas sp
Chávez Pasco Gaudhy Sujhey, Villanueva Aguilar Carlos Eduardo
Abstract
There is a growing problem around mining contamination by tailings in the Peruvian territory, cyanide
is used in mining and represents a poison for human life and the environment; In this sense, the present
investigation contributes in the search for strategies that allow the decontamination of cyanide. The
objective of the study is to evaluate the efficacy and the correlation of the pH of the culture medium
with the concentration of Sodium Cyanide in the degradation by Pseudomonas sp. In the methodology, a
bubble column bioreactor was used where pH values of 8, 9 and 10 were handled with cyanide
concentrations of 250ppm, 500ppm and 750ppm, the calculation of free cyanide was determined
volumetrically with AgNO3 and KI, the study variables were analyzed using the non-parametric
Spearman's Rho test. The results showed that the higher efficiency of cyanide (CN) degradation by
Pseudomonas sp. was 92.50% and this occurs at a pH of 8 and a concentration of 500 ppm of CN in the
medium, also observing a high strength of inverse correlation between the two variables of -0.949
through the coefficient of Spearman's rho.
Keywords
Biodegradation; Cyanide; Pseudomonas; pH; concentration
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