In this study, a microfluidic lab-on-chip sensor system that may enhance optical properties-based (fluorescence intensity) detection of liquid sample concentration is described. The microfluidic lab-on-chips sensors are Y shapes made of PMMA substrates by laser CO2 manufacturing techniques at power 60watt& travel speed of laser (250, 350 & 500) mm/s.The design of the sensing system includes optical fibers, reading from laser-induced fluorescence. It is suitable for low-cost, straightforward, & user-friendly sensing applications. The findings show that the addition of carbon nanotubes to a lower concentration made it feasible to assess improvements., as well as the concentrationrelated to fluorescence. Measurements were made using rhodamine B solutions in the range of 100-1.5625 mg/L, & carbon nano tubes (6.625,3.125,1.5625 mg/l).

Lab-on-chip, microfluidic, laser-induced fluorescence, surface roughness, rhodamine B.