Molecular Diagnosis of the Fungus Bipolaris Oryzae that causes Brown Spot Disease on Rice (Oryza Sativa)

Volume 20 No 6 (2022)

Ruaa Kamel Mahmoud, Sabah Latif Alwan , Wejdan Reda Mahmoud

DOI: 10.14704/nq.2022.20.6.NQ278

Abstract

This study was conducted to identify the genetic polymorphisms of Bipolaris oryzae that is isolated from rice crop in several portions of Al-Najaf city. One genetic locus covering a portion of the rRNA gene sequences were amplified in this study. A direct sequencing strategy was performed for the observed PCR amplicons in the amplified genetic locus. Subsequently, a comprehensive phylogenetic tree was constructed in the observed variants to assess the accurate phylogenetic distances alongside other relative fungal sequences

Keywords

Fungus, Bipolaris Oryzae, Brown Spot Disease, Rice, Oryza Sativa

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