New strains of Newcastle Disease Virus Detected by Analysis of a Fragment of the Fusion Gene for DNA Sequencing

Volume 20 No 10 (2022)

AlyaaMohson Reda, NafeaSabeeh Jasim

DOI: 10.14704/nq.2022.20.10.NQ55361

Abstract

The purpose of this study to analysis of a fragment of the fusion Gene for Newcastle disease virus by DNA sequencing. Three representative Fusion gene for Newcastle disease virus isolates were sequenced by Macrogen, Korea. Alignment of the sequences of the three isolated (AN02, AN03 and AN07) against the registered sequences of Newcastle viruses in the database of NCBI were studied, and the result showed that, 94% identity with avian orthoavulavirus 1 isolate ByCk/IR/MAME96/2017, 99% identity with avian orthoavulavirus 1 isolate INDTNC22020 and 86% identity with avian orthoavulavirus 1 isolate INDTNC22020 respectively.Virulent strain-specific motifs were found in the majority of circulating strains, as shown by partial sequencing of the F gene cleavage site.

Keywords

Fusion gene, Newcastle disease, DNA Sequencing

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