


Volume 20 No 20 (2022)
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Phytochemical investigation andAntimicrobial potential of a novel Polyherbal formulation
Syed Safiullah Ghori, Fouzia Tehseen , Mohd Shukhatulla Ansari ,Nadeem Fatima , Yousuf Hasan Khan ,Mohd Massarath , Tasleem Sultana and Hina Kauser
Abstract
In ayurveda, herbal formulations have been used for its medicinal and therapeutic applications since
ancient days. Plants are rich source of phytochemicals and have been explored for treatment of various
diseases. In the present investigation, antimicrobial evaluation of a novel polyherbal formulation(PHF)
containing of Trigonella foenum graecum, Cinnamomom verum, Aquilaria malaccensis, Azadirachta
indica, Nigella sativa and Carthamus tinctorious was done. Phytochemical investigation revealed the
presence of alkaloids, flavonoids, saponins, tannins, and glycosides. For antibacterial study, ofloxacin
was used as standard drug; gram positive bateria- Bacillus subtilis, Shigella dysenteria and
Staphyllococcus aureus and gram negative bacteria-Psuedomonas aeroginosa and Escherichia coli were
used. For antifungal activity, ketoconazole was used as standard drug; Candida albicans and Aspergillus
niger were used. The ethanolic extract of the PHF demonstrated better antimicrobial activity than the
petroleum ether extract. The zone of inhibition was strong for 100µg/ml ethanolic extract against gram
positive, gram negative and fungi strains. The PHEE3 showed significant activity of 21±0.421 to
26.83±0.404mm, then by PHEE3 ranging between 20.5±0.763 to 26.1±0.477 mm against all strains of
microbes used suggesting synergistic action by the plants used. Presence of phytochemicals like tannins,
flavonoids and other hydroxylated polyphenols, formed as secondary metabolites may be responsible
for the antimicrobial activity of the PHF.
Keywords
ayurveda, polyherbal formulation, phytochemical investigation, antibacterial, antifungal.
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