Background : During the storage period, erythrocytes would undergo biochemical changes known as storage lesions. Storage injury might lead erythrocytes to undergo hemolysis, impairing the PRC's quality, safety, and efficacy for recipients. The biochemical changes that occur were an increase in plasma potassium levels associated with cold storage temperatures causing the sodium/potassium exchange pump to became inactive and a decrease in pH-related 2,3-diphosphoglycerate (2,3-DPG) levels. This study sought to investigated the effect of storage duration on PRC quality by analyzing changes in potassium and 2,3-DPG levels on days 1, 10, 20, and 30 of storage. Method : This study used an observational analytical method with a time series design. There was a total of 50 PRC bags utilized as the samples. Potassium levels and 2,3-DPG were measured on day 1, day 10, day 20, and day 30. The study data were statistically analyzed using repeated measure ANOVA. Conclusion : According to the findings of a repeated measures analysis of variance performed on the levels of potassium and 2,3-DPG after 30 days of storage, there was a statistically significant difference (p < 0.00) in the levels of potassium and 2,3-DPG between day 1, day 10, day 20, and day 30

Packed red cells, Storage Lesion, Potassium, 2,3-DPG, Health Service