The only organisms capable of creating plant-associated metabolites and their therapeutically useful analogs are endophytic ones. Screening of all isolated endophytes, which could number in the hundreds, is required to find the prospective endophytic isolates producing bioactive chemicals. The technique of isolating endophytic organisms is comparatively laborious; nonetheless, screening the isolated fungi for the production of necessary metabolites is a laborious process. Genes that are involved in the full biosynthetic pathway may be found in endophytic organisms that produce compounds associated with plants. The goal of the current study was to look into the endophytic diversity of the Mysore region's native medicinal plant, Azadirachta indica. The morphology of the reproductive organs and spores served as the basis for conventional identification. several of those were categorized as mycelia sterilia because they lacked reproductive structures or distinguishing characteristics, while several endophytes could not be classified at the genus or species level. Bark samples had the highest rates of endophytic fungus colonization and isolation among the plant sections, followed by leaf samples.

Endophytes, Azadirachta indica, Piper nigrum, PGPR, Biochemical test.