Fruit pods of Prosopis cineraria was extracted with hot methanol and fractionated into different solvents. These extracts/fractions were used to evaluate various phytochemicals, total phenolic content, total flavonoids content, mineral content and protein binding capacity from pod part. Various phytoconstituents like saponins, tannins, carbohydrates, flavonoids, alkaloids, cardiac glycosides, fat and fixed oil and terpenoids have been screened from methanolic extract of Prosopis cineraria pods. Phytochemical investigation resulted the isolation of 1-heptatricontanol, 3, 8, 8-trimethoxy-3-piperidyl2, 2’ bimaphthalene-1, 1’, 4, 4’-tetrone, β-sitosterol and catechin. Total phenolics content was highest in methanol fraction (844.83 ± 0.66 mg GAEg-1) while total flavonoids content was highest in acetone fraction (608.31 ± 0.98 mg CEg-1). Prosopis cineraria posses highest Nitrogen (3125 ± 0.20 mg/100g) in acetone fraction followed by Potassium content (833 ± 0.32 mg/100g) in ethyl acetate fraction, Phosphorus content (675 ± 0.09 mg/100g) in aqueous fraction, Iron content (253.40 ± 0.02 mg/100g) in ethyl acetate fraction, Copper content (91.45 ± 0.02 mg/100g) in benzene fraction, Zinc content (21.94 ± 0.02 mg/100g) in methanol fraction and Manganese content (04.30 ± 0.01 mg/100g) in aqueous fraction. Acetone fraction was found to be maximum protein binding capacity at highest concentration, i.e. 8.30 ± 0.05%. The presence of active phytoconstituent with antioxidant properties may provide generous basis for the use of these plants in pharmaceutical industry

Prosopis cineraria (L) Druce, phytochemicals, phenolics, flavonoids, mineral content