Volume 20 No 9 (2022)
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Quantitative analysis for detection of cholesterol using colorimetric platform
Sangeeta Palekar, Bhavishya Waghwani, Huzefa Essaji, Jayu Kalambe
Abstract
For blood serum analysis in the human body, a miniaturized platform was designed in order to produce a useful, affordable, and simple-to-use healthcare system. The current effort intends to construct a platform that can perform all types of clinical blood serum analysis by applying colorimetric tests and detecting the absorbance of light that passes through the tested sample. The sample's cholesterol content was examined in order to assess and confirm the platform's use. For both known and unidentified samples, cholesterol detection was carried out throughout a linearity range of 1 mg/dL to 1000 mg/dL. The created colorimetric and optical platform has the benefits of being easily accessible, useful, affordable, resilient, requiring a small amount of reagent, and not requiring highly specialized labor. Due to the stability, usage of powerful processing units, and incorporation of cutting-edge electrical mechanisms, the quantitative analysis time was slashed to thirty seconds, enabling quick inspection and great throughput. Thus, the provided framework might be a great complement to the existing resource area as a general platform for every clinical analysis by just changing the reagents and wavelength filter
Keywords
Cholesterol, Blood; Colorimetric; Quantitative; Optical
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