This experiment was conducted in the saran-covered plastic house of the College of Agriculture and Marshes, ThiQar University, on 20.02.2022, to study the effect of spraying with glutathione and ascorbic acid on the physical and chemical properties of the grape plant Vitisvinifera L at one year age.The effect of glutathione compound at four concentrations (0, 75, 150, 225) mg.l-1 and ascorbic acid at four concentrations also (0, 50, 100, 150) mg.l-1 , and the interaction between them was studied and implemented as a factorial experiment with twofactors(4×4×3) by following the randomized complete block design (RCBD) with three replications (48 seedlings per replicate). The averages were tested using the least significant difference L.S.D at the probability level of 5%.The most important results obtained indicate that the effect of spraying with glutathione significantly on the studied vegetative characteristics, where the spraying treatment (G3) with a concentration of (225 mg l-1 ) was significantly superior to the control treatment (G0) in (plant height, leaves number, leaf area), where it gave the highest averages, respectively (99.637 cm, 34.427 leaves. plant-1 , 118.772 cm2 ). As for the leaves carbohydrate content, it amounted to (18.483%), while the values of the leaves nutrients content (nitrogen, phosphorous) (0.283%, 1.275%) respectively.As for ascorbic acid, it had a significant effect on the studied vegetative characteristics, the spraying treatment (C3) with a concentration of (150 mg l-1 ) was superior to (plant height (cm), leaves number (leaf plant-1 ), leaf area (cm2 ). Where it gave the highest averages, respectively (98.991, 32.647, 112.533 and carbohydrates (18.259%), while the percentage of nitrogen and phosphorous reached (0.274%, 1.192%).As for the binary interaction (compound glutathione + ascorbic acid) at a concentration (225 mg l-1 + 150 mg l-1 ), the treatments showed apparent statistical differences, as the treatments excelled in vegetative and chemical characteristics (plant height, leaves number, leaf area, carbohydrates content of Leaves, nitrogen percentage, phosphorous percentage) had a significant effect on the comparison treatment.

grape seedlings (Vitisvinifera L.)