Diabetes mellitus is a prevalent metabolic disorder characterized by persistent hyperglycemia due to insulin resistance or deficiency. The search for novel antihyperglycemic agents has led to the exploration of bioactive compounds derived from natural sources, including freshwater algae. This study focuses on the isolation, characterization, and antihyperglycemic evaluation of Ent-15-hydroxy-labda-8,2-ene,13 ediene-3-one, a terpenoid extracted from freshwater algae collected from Devtal and Gangasagar Lakes in Jabalpur, India. The compound was purified using column chromatography and HPLC, and its structure was confirmed through UV-Vis, FT-IR, Mass Spectrometry (MS), and NMR analysis.The antihyperglycemic activity of the purified terpenoid was evaluated in alloxan-induced diabetic Swiss albino mice, with fasting blood glucose levels monitored over 14 days. The results demonstrated a significant reduction in blood glucose levels, comparable to the standard drug Glibenclamide, highlighting the therapeutic potential of the algae-derived compound.Additionally, the study assessed water quality parameters (pH, temperature, TDS, EC, DO, BOD, COD, and nutrient content) in Devtal and Gangasagar Lakes to determine their impact on algal bioactivity. The findings underscore the importance of water quality management in sustaining bioactive compound production. This research contributes to the growing field of natural product-based diabetes management and emphasizes the need for further clinical studies and sustainable environmental practices for enhanced algal metabolite production.

Freshwater algae, antihyperglycemic activity, terpenoid, diabetes mellitus, bioactive compounds, Ent-15 hydroxy-labda-8,2-ene,13-ediene-3-one, chromatography, spectroscopy, water quality, alloxan-induced diabetes.