Treatment of 1-acetyl-4-(4-hydroxy phenyl) piperazine with aromatic or substituted aromatic aldehydes in presence of methanol and potassium hydroxide, formed 3-substituted phenyl-1--(4-(4- hydroxyl phenyl)- piperzin-1-yl)-Prop-2-en-1-one derivatives (RC-1 to RC-10). These Chalcones were used for condensation with guanidine hydrochloride to obtain pyrimidines. These are assayed for their antibacterial activity against Bacillus pumilus, Bacillus subtilis, Escherichia coli, and Proteus vulgaris; for antifungal activity against Aspergillus niger, Candida albicans strains. Antibacterial assay revealed that Bacillus subtilis and Proteus vulgaris were the most sensitive bacterial strains to compounds RC-2, 3, 4, 7, 10 and in the antifungal assay, compounds RC-1, 3, and 10 were highly effective against Aspergillus niger when compared to other investigated strains. The Pyrimidines were tested for anti Oxidant activity by Nitric Oxide radical scavenging method, measuring UV absorbance of solution of compound in Griess reagent and Super oxide radical method. Both the methods replicated the same results. Especially the compounds RP – 6, 9, 02 and 05 showed significant results.

1-acetyl-4-(4-hydroxy phenyl) piperazine, chalcone, pyrimidine, aldehydes, potassium hydroxide, methanol, antibacterial activity, antifungal activity, antioxidant activity.