Heavy metal pollution is rapidly explored due to industrialization and urbanization. Heavy metal pollutants are matter of concern because of their potential impact on flora and fauna. For our study, we collected seeds of Thevetia peruviana plant from industrially polluted region in pali district, arid zone of Rajasthan. Heavy metals such as Zn, Mn, Co, Pb, Cd, Fe, Ni, Cu, and Cr were accumulated by plants from the industrial waste. The MP-AES technique was used to analyse heavy metals by digested seed oil. The physicochemical characteristics of Thevetia peruviana seeds oil were determined such as oil yield 61.20%, specific gravity 0.8830, moisture content 1.38%, saponification value 125 mg KOH/L, acid value 1.07 mg KOH/L, iodine value 76.12 g of I2/100g and free fatty acid value 0.55 mg KOH/g. Preparation of FAME was carried out by trans esterification of Thevetia peruviana seed oil and then the fatty acid composition was determined using the GC-FID technique. The most predominant fatty acids in the Thevetia peruviana were Oleic acid 32.75%, Linoleic acid 31.46%, Palmitic acid 25.26% and least were Stearic acid 5.71% and Arachidic acid 1.36% respectively. The ratio of unsaturated fatty acids in Thevetia peruviana seed oil were high about 65.93 %, indicates that Thevetia peruviana was an excellent source for oleochemicals such as soaps, shampoos and biodiesel.

Thevetia peruviana, FAME, GC-FID, Heavy metals, MP-AES