Introduction : Dengue virus is a positive stranded RNA virus belonging to genus Flavivirus and family Flaviviridae . Dengue virus can infect infants, young children and adults. Dengue fever is an emerging arboviral public health problem in a large endemic population in the tropical and sub-tropical areas of the world, with varying degrees of clinical presentation. Aim and Objective: This study characterizes dengue cases and to find out the clinical profile along with the cytokines. Material and methods: This was a cross-sectional study carried out in the Department of Microbiology at a tertiary care centre for a period of 12 months i.e, January 2023 to December 2023. Prevalence was estimated by analyzing data from the results of IgM antibody by ELISA tests and variations in disease reporting by gender, age, serotypes, clinical manifestation and season were assessed. Results: In the present study a total of 450 samples were analyzed during the study period. Out of which 359 samples were found positive for dengue infection. Age group of 11–20 years was found to be the most infected by Dengue in which males are more commonly infected than females. Of those 4 Serotypes in overall samples, 39.55 % were caused by DENV-2, 30.64 % by DENV-3, 21.44 % by DENV-1, and 8.35% by DENV-4. The prevalence of Dengue was from August to November might be due to the rainy season. Clinical manifestation fever was observed to be 100%, headache 43.17%, and abdominal pain 44.01% was found in most of the dengue confirmed positive patient while altered sensorium 19.77% and positive tourniquet test 15.32% least found in patients . Conclusion: The present study showed that dengue infection was more prevalent among young males. Awareness and educating the community regarding mosquito breeding habits, prevention, and control of the vector will help in controlling the dengue. Development and use of an effective vaccine will be beneficial in endemic areas to help and limit the spread of infection.

Serotypes, DEN, IgM, ELISA, cytokines