The experiment was conducted in the plastic house belonging to Al-Mussaib Technical College, AlFurat Al-Awsat Technical University for the autumn season (2020) and the spring season (2021), to study the effect of bio-fertilizers and spraying with vitamins on the growth and flowering of Iris sp Sapphire Beauty cultivar. The experiment included two factors, the first factor included three levels of bio-fertilizers without addition (control treatment) which is symbolized by (S1), Azotobacter SPP bacteria which is symbolized by (S2), and Bacillus spp bacteria which is symbolized by (S3). As for the second factor, it included spraying with vitamins at three levels, without spraying (control treatment) which is symbolized by (F1), spraying with ascorbic acid at a concentration of (200 mg.L-1 ) which is symbolized by (F2), and spraying with thiamine at a concentration of (200 mg.L-1 ) which is symbolized by (F3). The factorial experiment was conducted according to the Randomized Complete Blocks Design (R.C.B.D) with three replicates and the averages were compared according to the least significant difference (L.S.D) test under the probability level of 5%, and the results were as follows: The results showed that Azotobacter (S2) treatment was significantly excelled on the rest of the other treatments by giving it the highest average Plant height 75.49 cm, Leaves area 1160.42cm 2 , Number of flowers 2.57 flower.plant-1 , Flower diameter 110.18 mm, Vase Life 10.73 Day,the spraying treatment with ascorbic acid at a concentration of (200 mg.L-1 ) was significantly excelled on the rest of the other treatments by giving it the highest values for the traits of Plant height 69.43cm, Leaves area 971.26cm 2 , Number of flowers 2.38 flower.plant-1 , Flower diameter 101.54 mm, Vase Life 10.22 Day.The bi- interaction treatment consisting of (Azotobacter bacteria + spraying with ascorbic at a concentration of (200 mg.L-1 ) has excelled on Plant height 79.45cm, Leaves area 1218.96 cm 2 , Number of flowers 3.62 flower.plant-1 , Flower diameter 117.70 mm, Vase Life 11.42 Day.

bio-fertilizer, vitamins, Iris plant