Food is essential for organisms to survive. Food preservation is a necessary process for keeping food safe. There are several difficulties with it, and adopting conventional techniques of food preservation would only exacerbate these issues and their adverse effects. Probiotics have been used as a last resort. One of the several types of bacteria used as probiotics is lactic acid bacteria (LAB). It creates a variety of antimicrobial substances, including antifungal ones .Therefore , secondary products of this local LAB isolates (Pediococcus sp., Lactobacillus sp., and Enterococcus sp .) were extracted and synergistically with sesame oil. Aspergillus niger was treated with these extracts. The results of our study showed that there was an inhibitory activity for extracts of different bacterial isolates of LAB according to the type of those isolates, as well as there was a synergistic inhibitory activity of some bacterial extracts with sesame oil and that varies to the species of isolates, while some of isolates of LAB did not show any effectiveness, whether synergistic or otherwise, against Aspergillus niger.There was the highest synergy antifungal activity of Lactobacillus sp L9 extract with sesame oil at 8000% and a synergistic growth area reached 63.58 mm2 followed by extract of Lactobacillus sp L5 (isolated from milk) synergistic with sesame oil with a synergy inhibitory activity against A.niger estimated at 2780% with a synergistic growth area 113.04 mm2 ,while Pediococcussp L3, Lactobacillus sp L4 ,Pediococcussp L6 and Enterococcus faecalis L8 did not show any inhibitory effect . whether synergistic or otherwise , against A.niger

Lactic Acid Bacteria , sesame oil , synergistic activity , antifungal activity