The atmospheric pressure cold plasma jet (APCPJ) is a promising approach for sterilizing and eradicating pathogenic bacteria in a natural setting.A helium-APPJ was created by a single electric discharge and used to inhibit pathogenicStaphylococcus aureus(pS.aureus)as a nosocomial pathogenic model microorganism in this study.Discharge characteristics (including waveform and frequency of applied voltage), jet properties (such as feed gas flow rate, jet length, thermal effect, and optic emission spectra), and in vitropurging performance (in terms of clear/inhibition area anddiameter of a pellucid zone(DPZ)) were investigated.A homogeneous helium plasma jet was generated in an energy-saving method at (13kHz, 4kV), (2 slm) with a jet (10mm)at 37C0.The APCPJ used helium gas through a high-voltage electrical spark to achieve a good performance of the target pS.aureus strain repression tool during various treatment periods (2, 4, 6, 8, 10 min).The optical emission spectra indicate that active species such as excited particles, ions, and radicals produced from the generation of cold helium plasma jets played an essential role in the sterilization processes.The traditional antibiogram for sensitivity was done by disk diffusion test, the pS.aureus showed high resistance to Erythromycin, Methicillin,and Gentamycin, with a DPZ(0, 7,and 10mm) respectively, and intermediate resistance toCiprofloxacin(26mm-with many dispersed colonies grown within the inhibition zone).According to the DPZ, 10 min was the best exposure time significantly, with 32mm for the inhibition action than the rest also, 4, 6, and 8 mins were good exposure periods capable of inhibitingpSaureus without any significant difference among them were (24,26, and 27)mm respectively.This study concluded that APCPJ could be dependent as a novel unique alternative physiotherapeutic device and/or can assist and boost the efficiency of chemical and biological treatment in the different medical management fields.

Cold Plasma Jet, APPJ, Helium Gas,Physio-Medical, Staphylococcus Aureus