In herbal medicine systems like Indian Ayurveda, Traditional Chinese Medicine, and Indonesian Jamu, P. niruri has a lengthy history. Dysentery, influenza, vaginitis, tumors, diabetes, diuretics, jaundice, kidney stones, and dyspepsia are among the ailments for which the whole plant is used as a remedy. The present study involved extraction of Phyllanthus niruri leaves via various solvents (petroleum ether, chloroform, acetone, ethanolic, and aqueous), determination of physicochemical evaluation, estimation of ash values, and phytochemical evaluation (sugars, alkaloids, glycosides, tannins, flavonoids, steroids, proteins and terpenes). Partial characterization of phytoconstituents in bioactive fractions was done using column chromatography as well as thin-layer chromatography (TLC). The total ash values were found to be 6.5% w/w which indicating that the considerable presence of inorganic matter. The acid insoluble ash values were found to be 2.5% w/w whereas the water soluble ash values were found to be 1.2% w/w. The phytochemical studies have shown that acetone, ethanol, and aqueous extracts of leaves of P. niruri L. contains almost same and maximum number of pharmacologically active constituents; i.e., glycosides, sponins, phytosterol and flavonoids. Through column chromatography, on recurrent purifying using methanol, the large band-6, and acetone: methanol (50:50), yields the colorful chemical phyllanthin. Through TLC, the elution using n-hexane, ethyl acetate, and formic acid in the ratio of 3.1:1.4:0.5 v/v/v and demonstrated an Rf value of 0.6. The obtained data and observations for this pharmacologically active plant will positively open new avenues for futuristic research and will definitely serve as a reference for pharmacognosists for further exploration

Phyllanthus niruri, Extraction, Phytochemical, Phytoconstituents, Physiochemical, Partial characterization